Student feedback and possible modifications to the exercise as well as supplemental exercises are also discussed. The degree of sequence similarity between the aligned proteins allows students to verify their success in processing the gene, as well as to visualize the truncated protein product from the Tc5 mutant allele. MegAlign®, the second program, aligns the wild‐type sequence of the UNC‐22 protein and the wild‐type and mutant protein sequences that were constructed.
#Editseq software software#
The open reading frames of both cDNAs are located and then translated. EditSeq software analysis showed that the 567 nucleotides of the canine tetherin coding sequence (CDS) encode 188 amino acids (aa) whose total molecular weight is 20,626.84 daltons.
The animals involved include mammals, birds, reptiles and fish. Device Type: Digital Camera Supported OS: Win 2003 Server, Win XP Home, Win XP Pro, Win 2000 Workstation, Win 2000 Server, Win NT 4.
#Editseq software driver#
The accession numbers of the sequences used in Genbank are shown in Table 2. Editseq Software Windows 10 To See Discuss driver problems and ask for help from our community on our.
#Editseq software download#
The unc‐22(r466) allele is generated by inserting the sequence of the transposon Tc5 into the appropriate location of the third exon in unc‐22. Download the coding region sequence of IRF4 of other animals from the NCBI website, use Editseq software to convert it into a text file, and use the neighbor-joining algorithm in MEGA5.1 to construct the phylogenetic tree. The first program, EditSeq®, enables students to experience the meticulous process required to precisely locate and remove intron sequences from the wild‐type unc‐22 allele to generate a cDNA sequence. The nucleotide sequences are manipulated using two programs in the Lasergene® software package from DNASTAR®. You can also utilize the integrated internet interface to search NCBI's. Students search GenBank for the wild‐type nucleotide sequence of the Caenorhabditis elegans unc‐22 gene, the amino acid sequence of its gene product, and the nucleotide sequence of the transposon Tc5. EditSeq enables you to work on nucleic acid and protein sequences of all sizes from a wide variety of popular formats. The software does not provide the aligning function, so you should do the alignment outside, or use highly-similar sequences. In addition, the activity illustrates the severe effect mutations in a gene sequence can have on the protein product. We have developed an introductory bioinformatics exercise for sophomore biology and biochemistry students that reinforces the understanding of the structure of a gene and the principles and events involved in its expression.
An introductory bioinformatics exercise to reinforce gene structure and expression and analyze the relationship between gene and protein sequences An introductory bioinformatics exercise to reinforce gene structure and expression and analyze.Īlmeida, Craig A.